Hygromycin B ReadyMade™ Solution

SKU: H011-20ml

Key Benefits

ReadyMade™ formulation for immediate use, eliminating preparation steps and ensuring consistent performance
High selection stringency for efficient elimination of non-resistant cells
Compatible with hph-based selection systems
Effective across mammalian cells, yeast, and plant systems
High consistency for reproducible experimental outcomes

OptionAvailabilityPrice

Size(ml): 20Out of Stock€ 99,90
Size(ml): 100SKU: H011-100mlOut of Stock€ 99,90

Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus and widely used as a selective agent in eukaryotic cell culture and plant transformation. It exhibits strong activity against bacteria, fungi, and eukaryotic cells by inhibiting protein synthesis. Hygromycin B is commonly used for the selection and maintenance of genetically engineered cells expressing the hygromycin resistance gene (hph), making it a key reagent for stable cell line generation and transgenic plant development. Mechanism of Action: Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action of Neomycin, Gentamicin, and G418.

Product Details

Property	Value
Duchefa No.	H0192
Primary Category	Research+Reagents
Secondary Category	Selection Agent
Key Feature	Stable selection
CAS.NO.	31282-04-9
Solvent	PBS Buffer
Concentration	50 mg/ml

Note

For research use only. Not for diagnostic or therapeutic use.

Safety Data Sheets

H011 Hygromycin B Solution A0Download PDF

Related Products

Validamycin A ReadyMade™ Solution

Validamycin A is an aminoglycoside-derived antifungal agent produced by Streptomyces hygroscopicus. It is widely used in plant biology and agricultural research for its activity against phytopathogenic fungi, particularly Rhizoctonia solani. Validamycin A is commonly applied as a tool to study carbohydrate metabolism and fungal growth regulation. Mechanism of Action: Validamycin A inhibits trehalase, an enzyme responsible for the hydrolysis of trehalose into glucose. This inhibition disrupts energy metabolism in fungi, leading to abnormal hyphal growth and suppression of fungal development.

20 · € 99,90

Trimethoprim Lactate ReadyMade™ Solution

Trimethoprim lactate is a synthetic diaminopyrimidine antibiotic widely used in microbiology and molecular biology research. It exhibits antibacterial activity by selectively inhibiting bacterial dihydrofolate reductase (DHFR), a key enzyme in folate metabolism. Trimethoprim is commonly used alone or in combination with sulfonamides such as sulphamethoxazole to achieve synergistic inhibition of bacterial growth. Mechanism of Action: Trimethoprim selectively inhibits bacterial dihydrofolate reductase (DHFR), preventing the reduction of dihydrofolic acid to tetrahydrofolic acid. This disrupts the synthesis of nucleotides and ultimately inhibits DNA, RNA, and protein synthesis. When combined with sulfonamides, trimethoprim provides a sequential blockade of folate biosynthesis, resulting in enhanced antibacterial efficacy.

20 · € 99,90

Sulphamethoxazole ReadyMade™ Solution

Sulphamethoxazole is a sulfonamide antibiotic widely used in microbiology and molecular biology research. It exhibits broad-spectrum activity against Gram-positive and Gram-negative bacteria by inhibiting folate biosynthesis. Sulphamethoxazole is commonly used alone or in combination with trimethoprim (TMP-SMX) to achieve synergistic inhibition of bacterial growth. Mechanism of Action: Sulphamethoxazole is a structural analog of para-aminobenzoic acid (PABA) and competitively inhibits dihydropteroate synthase (DHPS), a key enzyme in folate synthesis. This blocks the production of dihydrofolic acid, leading to impaired DNA, RNA, and protein synthesis.

10 · € 99,90

Phleomycin ReadyMade™ Solution

Phleomycin is a glycopeptide antibiotic belonging to the bleomycin family, widely used as a selective agent in molecular biology and cell biology research. It exhibits potent activity against bacteria and eukaryotic cells by inducing DNA damage. Phleomycin is commonly used for the selection of genetically engineered cells expressing the Sh ble resistance gene, making it a key reagent in yeast, fungal, and mammalian expression systems. Mechanism of Action: Phleomycin binds to DNA and, in the presence of oxygen and metal ions, induces single- and double-strand breaks. This leads to inhibition of DNA replication and transcription, ultimately resulting in cell death.

20 · € 99,90

PPT (DL-Phosphinothricin) ReadyMade™ Solution

DL-Phosphinothricin (PPT), also known as glufosinate, is a broad-spectrum herbicidal compound widely used in plant biology and genetic engineering. It functions as a potent inhibitor of glutamine synthetase (GS), a key enzyme in nitrogen metabolism. PPT is extensively used as a selection agent in plant transformation systems, particularly in combination with the bar or pat resistance genes, making it a core reagent in transgenic plant development. Mechanism of Action: PPT inhibits glutamine synthetase, leading to accumulation of ammonia and disruption of nitrogen metabolism. This results in inhibition of photosynthesis and cellular metabolism, ultimately causing plant cell death.

20 · € 99,90

Mitomycin C ReadyMade™ Solution

Mitomycin C is a potent antitumor antibiotic isolated from Streptomyces caespitosus, widely used in molecular and cell biology research as a DNA crosslinking agent. It is commonly applied to study DNA damage response, cell cycle regulation, and apoptosis. Mitomycin C is also frequently used to inhibit cell proliferation in co-culture systems, such as feeder cell preparation. Mechanism of Action: Mitomycin C is a bioreductive alkylating agent that becomes activated intracellularly and forms covalent crosslinks between DNA strands. These crosslinks inhibit DNA replication and transcription, ultimately leading to cell cycle arrest and apoptosis.

1 · € 99,90

Methotrexate ReadyMade™ Solution

Methotrexate (MTX) is a folate analog and potent antimetabolite widely used in molecular and cell biology research. It inhibits dihydrofolate reductase (DHFR), a key enzyme in nucleotide biosynthesis, and is commonly used to study cell proliferation, DNA synthesis, and apoptosis. Methotrexate is also a critical reagent in DHFR-based gene amplification systems, particularly in CHO cell expression platforms for recombinant protein production. Mechanism of Action: Methotrexate inhibits dihydrofolate reductase (DHFR), preventing the conversion of dihydrofolate to tetrahydrofolate, which is required for thymidylate and purine synthesis. This leads to inhibition of DNA replication and cell division. In DHFR-based systems, methotrexate applies selective pressure that drives amplification of the DHFR gene and linked transgenes, enabling high-level protein expression.

20 · € 99,90

Mercaptopurine Monohydrate ReadyMade™ Solution

Mercaptopurine monohydrate (6-mercaptopurine, 6-MP) is a thiopurine analog widely used as an antimetabolite in molecular biology and cell biology research. It interferes with purine nucleotide metabolism and is commonly used to study DNA synthesis, cell proliferation, and apoptosis. Mercaptopurine is also used in genetic selection systems, particularly in combination with hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-based selection. Mechanism of Action: Mercaptopurine is converted intracellularly into active thioguanine nucleotides, which inhibit de novo purine synthesis and become incorporated into DNA and RNA. This disrupts nucleic acid synthesis and function, ultimately inhibiting cell proliferation and inducing cell death.

20 · € 99,90

Kanamycin Sulphate Monohydrate ReadyMade™ Solution

Kanamycin sulphate monohydrate is a broad-spectrum aminoglycoside antibiotic originally isolated from Streptomyces kanamyceticus. It is widely used in molecular biology, microbiology, and cell culture applications for bacterial selection and contamination control. Kanamycin is commonly employed as a selective agent in cloning systems utilizing the neomycin/kanamycin resistance gene (neo/kan), and is preferred for its higher stability compared to β-lactam antibiotics such as ampicillin. Mechanism of Action: Kanamycin binds to the 30S ribosomal subunit, causing misreading of mRNA and inhibition of protein synthesis. This results in the production of nonfunctional proteins and ultimately leads to bacterial cell death.

20 · € 99,90

8-Hydroxyquinoline ReadyMade™ Solution

8-Hydroxyquinoline is a heterocyclic organic compound widely used as a metal chelating agent and antimicrobial agent in biological research. It exhibits activity through its ability to bind metal ions, disrupting essential cellular processes. In plant biology, 8-hydroxyquinoline is commonly used as a mitotic inhibitor to accumulate cells at metaphase for cytogenetic analysis. Mechanism of Action: 8-Hydroxyquinoline forms stable complexes with metal ions such as iron and copper, interfering with metal-dependent enzymatic processes in microorganisms. In plant cells, it disrupts spindle formation during mitosis, leading to arrest at metaphase and facilitating chromosome observation.

20 · € 99,90